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RMRC No. RMRC13427 Order
Strain/Stock Designation NOD.Cg-PrkdcscidIl2rgem1Narl/Narl
Status
LM| (Live Animal)
Availability Level3
Pricing
Last Update Date 2024/7/9
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： COI (coisogenic strain),MSR (mutant strain)
Mutant Type ： TM (Target Mutation)
Other Names ： ASID
Background Strain ： NOD.CB17-Prkdcscid/Narl
Strain Description ：
1.  The severe immunodeficiency of this strain is suitable for engraftment of human hematopoietic stem cells (HSC), peripheral blood mononuclear cells (PBMC), patient-derived xenografts (PDX) or adult stem cells … humanized experiments.
2.  The expression of theIl2rg (interleukin 2 receptor subunit gamma) gene is absent in this strain by flow cytometry. The presence of natural killer (NK) cells is extremely deficient (~1.8 %).
3.  Based on the background strain NOD.CB17-Prkdcscid/Narl, this strain is deficient in T cells and B cells.
4.  This CRISPR/Cas9-generated mutant of the Il2rg gene carries a deletion mutation from the start codon to the stop codon.
5. 
The Il2rg deletion mutant allele was created using CRISPR/Cas9 endonuclease-mediated genome editing. Guide RNAs were selected to target exon 1 and exon 8 of the Il2rg gene on chromosome X.These guide RNAs and Cas9 nuclease was introduced into zygotes of NOD.CB17-Prkdcscid/Narl, and then transferred to pseudopregnant females. Progeny were screened by genotyping of the target region. The founder was backcrossed to NOD.CB17-Prkdcscid/Narl for germline transmission.
Depositor ：
National Laboratory Animal Center, National Applied Research Laboratories / 財團法人國家實驗研究院國家實驗動物中心
Email ： bujiii@narlabs.org.tw
Allele Symbol ： Prkdcscid
Allele Name ： protein kinase, DNA activated, catalytic polypeptide; severe combined immunodeficiency
Allele Description ：
Allele Symbol ： Il2rgem1Narl
Allele Name ： interleukin 2 receptor, gamma chain, endonuclease-mediated mutation, National Applied Research Laboratories
Strain of Origin ： NOD.CB17-Prkdcscid/Narl
Gene Symbol ： Il2rg
Gene Name ： interleukin 2 receptor, gamma chain
Chromosome ： X
Genotyping Method ：            
Genotyping NLAC ：            
Research Application ：
Cancer Immunology and Inflammation
Construction Technologies ： No
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： homo x homo
References ： No

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由國家實驗動物中心所提供
Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge NLAC-Narlabs
Construct Map ：
 
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