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RMRC No.

RMRC13393

Order

Strain/Stock Designation

B6.129-Prap1^tm1Yen/Narl

Status

EM| (Cryopreserved Embryos)

Availability

Level4

Pricing

(Embryos) 　International：560USD
(Cryo Recovery) 　International：760USD

Last Update Date

2023/7/11

Analysis Data

Description
Genes &
alleles
Phenotype
Health &
husbandry
References
Specific Term
and Conditions
Construct map

Species

：

laboratory mouse

Strain Type

：

MSK (mutant stock),CON (congenic strain)

Mutant Type

：

TM (Target Mutation)

Other Names

：

Background Strain

：

C57BL/6

Strain Description

：

1.	A targeting vector was designed to insert exon 3 ofPrap1 gene with lacZ reporter linked to a flanked neomycin resistance cassette. The vector was electroporated into R1 embryonic stem cells. Correctly targeted embryonic stem cells were injected into C57BL/6 blastocysts. The Parp1 knockout strain has been backcrossed to C57BL/6J for at least 10 generations.
2.	The epithelial-specific expression of Prap1 was completely ablated in this strain.
3.	The length of the small intestine in this strain was significantly longer than that in control mice.
4.	On a normal chow diet, the phenotype of this strain is as follows, 1) Prap1 knockout strain had adefect in lipid absorption. 2) PraP1 knockout did not affect apoB mRNA expression, but significantly less apoB48 and apoB100 and triglycerideswere distributed to the CM/VLDL-rich fraction of Prap1 knockout plasma compared withthesame fraction from control mouse plasma.
5.	On a high-fat diet, the phenotype of this strain is as follows, 1) Gained significantly less body weight compared with control mice. The leaner phenotype of Prap1 knockout mice was due to defect on lipid absorption. 2) Fat mass percentage of the knockout mice was significantly reduced and both adipose tissues (including iWAT and eWAT) and adipocytes were smaller than control. Significant decrease in the steady state plasma level of triglyceride and phospholipid. 3) Feces of knockout mice contained more lipids than control mice. 4) The lipid droplets, hepatocytes with vacuolation and hepatic triglyceride contents were s significant reduced; manifesteddiminished high-fat diet-induced hepatosteatosis.
6.	Fertility is less efficient than control mice.

Depositor

：

Hsin-Fang Yang-Yen

楊性芳

：

imbyy@gate.sinica.edu.tw

Allele Symbol	：	Prap1^tm1Yen

Allele Name	：	proline-rich acidic protein 1, target mutation 1, Hsin-Fang Yang-Yen

Allele Description	：






Strain of Origin	：	129

Gene Symbol	：	Prap1

Gene Name	：	proline-rich acidic protein 1

Chromosome	：	7






Genotyping Method	：	Download
Genotyping NLAC	：

Research Application

：

Cardiovascular	Metabolism
Diabetes and Obesity

Construction Technologies

：

LacZ

Animal Health Reports	：

Colony Maintenance Breeding & Husbandry	：

Colony Maintenance Mating System	：	hetero/hemi x hetero/hemi

References

：

PubMed ID: 33168624

Specific Terms of Use

：

有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： J Biol Chem. 2020 Nov 9:jbc.RA120.015002. doi: 10.1074/jbc.RA120.015002

Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： J Biol Chem. 2020 Nov 9:jbc.RA120.015002. doi: 10.1074/jbc.RA120.015002

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由楊性芳研究員所提供

Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge Dr. Hsin-Fang Yang-Yen

分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: imbyy@gate.sinica.edu.tw

Construct Map

：