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RMRC No. RMRC13350 Order
Strain/Stock Designation B6.129S6-Hspb7tm1(FLAG)Yty/Narl
Status
EM| (Cryopreserved Embryos)
Availability Level4
Pricing (Embryos) 　International：560USD
(Cryo Recovery) 　International：760USD
Last Update Date 2018/5/11
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： MSK (mutant stock),CON (congenic strain)
Mutant Type ： TM (Target Mutation)
Other Names ：
Background Strain ： C57BL/6
Strain Description ：
1. A targeting vector was generated in which exons 2 and 3 of the Hspb7 gene wereloxp-flanked and a FLAG-tag sequence inserted before the stop codon of the Hspb7 gene. The construct was electroporated into 129S6/SvEvTac -derived TC1 embryonic stem (ES) cells.Blastocyst injection of ES cells carried targeted allele was used to obtain chimera. The chimeric males were backcrossed to C57BL/6 females. The backcrosses involved mice constitutively expressing the FLP recombinase to remove the neomycin cassette from targeted allele.
2. The offspring of muscle specific-Hspb7 gene knockout began to die at the age of postnatal 1 week. The survival curve showed a wide spectrum of lethality in muscle specific-Hspb7 gene knockout mice as shown in reference.
Depositor ：
Yu-Ting Yan / 顏裕庭
Email ： yyan@ibms.sinica.edu.tw
Allele Symbol ： Hspb7tm1(FLAG)Yty
Allele Name ： heat shock protein family, member 7 (cardiovascular), target mutation 1, Yu-Ting Yan
Allele Description ：
Strain of Origin ： 129
Gene Symbol ： Hspb7
Gene Name ： heat shock protein family, member 7 (cardiovascular)
Chromosome ： 4
Genotyping Method ： Download            
Genotyping NLAC ：            
Research Application ：
Cardiovascular Cell and Developmental Biology
Construction Technologies ：
Cre-lox system FLP-FRT system
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： hetero/hemi x wild type
References ： PubMed ID: 26929074

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： HSPB7 interacts with dimerized FLNC and its absence results in progressive myopathy in skeletal muscles. J Cell Sci. 2016 Apr 15;129(8)
Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： HSPB7 interacts with dimerized FLNC and its absence results in progressive myopathy in skeletal muscles. J Cell Sci. 2016 Apr 15;129(8)
分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: yyan@ibms.sinica.edu.tw
Construct Map ：
 
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