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RMRC No. RMRC13440 Order
Strain/Stock Designation C57BL/6-Apoeem1(APOE*4, APOE*2)Narl/Narl
Status
SP| (Cryopreserved or freeze-dried sperm)
Availability Level4
Pricing Industry：23,680NTD　Academia: 23,680NTD　NSTC: 16,580NTD
Last Update Date 2026/2/23
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： COI (coisogenic strain),IS (inbred strain)
Mutant Type ： TM (Target Mutation)
Other Names ： APOE4,2 Knockin
Background Strain ： C57BL/6JNarl
Strain Description ：
1.  The original construct was designed to achieve a functional humanization of the Apoe locus via CRISPR/Cas-mediated knock-in. The design intended to replace mouse exons 3 and 4. A human APOE4 coding sequence followed by an IRES-driven inducible Dre recombinase.An additional human APOE4 cistron regulated by Tet-On 3G promoter is inserted behind the mouseApoe gene. These two APOE4 genes mentioned above were flanked by the rox elements. A human APOE2 gene followed by 3XFlag Tag is the downstream of the rox element.
2.  The primary transcript of humanized APOE4 allele has been validated at the mRNA level by RT-PCR.
3.  Activation of Dre recombinase by tamoxifen is expected to mediate deletion between two rox elements and consequently, leading to conversion of human APOE4 expression to APOE2 expression.
Depositor ：
National Center for Biomodels, National Institutes of Applied Research / 財團法人國家實驗研究院國家生物模式中心
Email ： bujiii@narlabs.org.tw
Allele Symbol ： Apoeem1(APOE*4, APOE*2)Narl
Allele Name ： apolipoprotein E,endonuclease-mediated mutation 1(APOE*4, APOE*2),National Applied Research Laboratories
Allele Description ：
Strain of Origin ： C57BL/6JNarl
Gene Symbol ： Apoe
Gene Name ： apolipoprotein E
Chromosome ： 7
Genotyping Method ：            
Genotyping NLAC ：            
Research Application ：
Cardiovascular Metabolism
Neurobiology Research
Construction Technologies ：
Tet Expression system DrePBD (RU486 inducible Dre recombinase) 、rox、3XFl
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： homo x homo
References ： No

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由NCB, NIAR所提供
Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge NCB, NIAR
Construct Map ：
 
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