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RMRC No. RMRC13439 Order
Strain/Stock Designation C57BL/6-Apoeem1(APOE*4)Narl/Narl
Status
SP| (Cryopreserved or freeze-dried sperm)
Availability Level4
Pricing Industry：23,680NTD　Academia: 23,680NTD　NSTC: 16,580NTD
Last Update Date 2026/2/23
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： COI (coisogenic strain),IS (inbred strain)
Mutant Type ： TM (Target Mutation)
Other Names ： APOE4 Knockin
Background Strain ： C57BL/6JNarl
Strain Description ：
1.  The original construct was designed to achieve a functional humanization of the Apoe locus via CRISPR/Cas-mediated knock-in. The design intended to replace mouse exons 3 and 4. A human APOE4 coding sequence followed by an IRES-driven inducible Dre recombinase.An additional human APOE4 cistron regulated by Tet-On 3G promoter is inserted behind the mouseApoe gene. These two APOE4 genes mentioned above were flanked by the rox elements. A human APOE2 gene followed by 3XFlag Tag is the downstream of the rox element. Only rox-flanked two APOE4 cassettes were successfully knocked into the designated Apoe locus.
2.  This strain can only express human APOE4 protein without endogenous mouse Apoe expression. It serves as a functional human APOE4 knock-in mouse model.
3.  The IRES-mediated protein translation including the expression and functional activity of the inducible Dre recombinase as well as Tet-on 3G transactivator has not been evaluated.
Depositor ：
National Center for Biomodels, National Institutes of Applied Research / 財團法人國家實驗研究院國家生物模式中心
Email ： bujiii@narlabs.org.tw
Allele Symbol ： Apoeem1(APOE*4)Narl
Allele Name ： apolipoprotein E,endonuclease-mediated mutation 1,National Applied Research Laboratories
Allele Description ：
Strain of Origin ： C57BL/6JNarl
Gene Symbol ： Apoe
Gene Name ： apolipoprotein E
Chromosome ： 7
Genotyping Method ： Download            
Genotyping NLAC ：            
Research Application ：
Cardiovascular Metabolism
Neurobiology Research
Construction Technologies ：
Tet Expression system
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： homo x homo
References ： No

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由NCB, NIAR所提供
Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge NCB, NIAR
Construct Map ：
 
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