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RMRC No. RMRC13398 Order
Strain/Stock Designation C57BL/6-Lama3em1Tmh/Narl
Status
EM| (Cryopreserved Embryos)
Availability
Pricing (Embryos)  Industry:11,105NTD Academia: 7,775NTD NSTC: 5,415NTD
(Cryo Recovery)  Industry:20,815NTD Academia: 13,880NTD NSTC: 13,880NTD
Last Update Date 2023/8/21
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species laboratory mouse
Strain Type COI (coisogenic strain),MSR (mutant strain)
Mutant Type TM (Target Mutation)
Other Names
Background Strain C57BL/6
Strain Description
1. 
The 488th nucleotide C in Exon 1 was replaced to T (CAG to TAG) by CRISPR/CAS9 technology and introduced the Q95X point mutation into exon 1 of the lama3 gene.
Correctly targeted pups were identified by PCR and further bred to C57BL/6JNarl to develop the colony.
Depositor
Meng-Han Tsai / 蔡孟翰
Email menghan@cgmh.org.tw
Allele Symbol
Allele Name
Allele Description
Allele Symbol Lama3em1Tmh
Allele Name laminin, alpha 3, endonuclease-mediated mutation 1,Meng-Han Tsai
Strain of Origin C57BL/6
Gene Symbol Lama3
Gene Name laminin, alpha 3
Chromosome 18
Genotyping Method Download            
Genotyping NLAC            
Research Application
Neurobiology Research
Construction Technologies No
Animal Health Reports
Colony Maintenance
Breeding & Husbandry
Colony Maintenance
Mating System
hetero/hemi x wild type
References No

Specific Terms of Use
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果,不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時,應揭露並致謝本材料係由蔡孟翰所提供
Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge Meng-Han Tsai
分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時,應附上寄存人簽名之授權分讓同意書作為附件;NLAC- RMRC 在取得寄存人書面同意後,始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: menghan@cgmh.org.tw
Construct Map
 
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