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RMRC No.

RMRC13394

Order

Strain/Stock Designation

C57BL/6-Prap1^em1(E85V)Yen/Narl

Status

EM| (Cryopreserved Embryos)

Availability

Level4

Pricing

(Embryos) 　Industry：11,105NTD　Academia: 7,775NTD　NSTC: 5,415NTD
(Cryo Recovery) 　Industry：20,815NTD　Academia: 13,880NTD　NSTC: 13,880NTD

Last Update Date

2023/7/11

Analysis Data

Description
Genes &
alleles
Phenotype
Health &
husbandry
References
Specific Term
and Conditions
Construct map

Species

：

laboratory mouse

Strain Type

：

COI (coisogenic strain),MSR (mutant strain)

Mutant Type

：

TM (Target Mutation)

Other Names

：

Background Strain

：

C57BL/6

Strain Description

：

1.	CRISPR/Cas9 endonuclease mediated genome editing introduced E85V into Prap1 gene by coinjection of a single-stranded mutant DNA oligo, Prap1-targeting sgRNA and Cas9 mRNA into pronulear stage one-cell C57BL/6J mouse embryos. Mice carried E85V mutation were bred in C57BL/6 background.
2.	The length of the small intestine in this strain was longer than littermate control.
3.	On a normal chow diet, the phenotype is as follows, 1) E85V mutant had adefect in lipid absorption 2) E85V mutantiondid not affect apoB mRNA expression, but significantly less apoB48 and apoB100 and triglycerideswere distributed to the CM/VLDL-rich fraction of E85Vplasma compared with thesame fraction from control mouse plasma.
4.	On a high-fat diet, the phenotype of this strain is as follows, 1) Gained significantly less body weight compared with control mice. 2) Manifested smaller adipose tissues (including iWAT and eWAT) and lowe triglyceride levels than controls. 3) The lipid droplets, hepatocytes with vacuolation and hepatic triglyceride contents were significant reduced;manifesteddiminished high-fat diet-induced hepatosteatosis
5.	Phenotype of E85V mutant is very similar to that of Prap1 deficient mice

Depositor

：

Hsin-Fang Yang-Yen

楊性芳

：

imbyy@gate.sinica.edu.tw

Allele Symbol	：	Prap1^em1(E85V)Yen

Allele Name	：	proline-rich acidic protein 1, endonuclease-mediated mutation 1, Hsin-Fang Yang-Yen

Allele Description	：






Strain of Origin	：	C57BL/6

Gene Symbol	：	Prap1

Gene Name	：	proline-rich acidic protein 1

Chromosome	：	7






Genotyping Method	：	Download
Genotyping NLAC	：

Research Application

：

Cardiovascular	Metabolism
Diabetes and Obesity

Construction Technologies

：

Animal Health Reports	：

Colony Maintenance Breeding & Husbandry	：

Colony Maintenance Mating System	：	hetero/hemi x hetero/hemi

References

：

PubMed ID: 33168624

Specific Terms of Use

：

有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： J Biol Chem. 2020 Nov 9:jbc.RA120.015002. doi: 10.1074/jbc.RA120.015002

Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： J Biol Chem. 2020 Nov 9:jbc.RA120.015002. doi: 10.1074/jbc.RA120.015002

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由楊性芳研究員所提供

Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge Dr. Hsin-Fang Yang-Yen

分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: imbyy@gate.sinica.edu.tw

Construct Map

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