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RMRC No. RMRC13388 Order
Strain/Stock Designation 129/Sv-Fgfr3tm1.1(FGFR3*)Ytc/Narl
Status
EM| (Cryopreserved Embryos)
Availability Level4
Pricing (Embryos) 　Industry：11,105NTD　Academia: 7,775NTD　NSTC: 5,415NTD
(Cryo Recovery) 　Industry：20,815NTD　Academia: 13,880NTD　NSTC: 13,880NTD
Last Update Date 2025/2/14
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： COI (coisogenic strain),MSR (mutant strain)
Mutant Type ： TM (Target Mutation)
Other Names ： ACH mice
Background Strain ： 129
Strain Description ：
1.  This mouse model offers a tool for assessing potential therapeutic approaches for skeleton dysplasias related to over-activation of human FGFR3.
2.  The targeting vector was designed to replace the mouse Fgfr3 with human FGFR3 cDNA carrying the ACH mutation (FGGR3G380R) under the full control of the endogenous mouse Fgfr3 promoter, intron 1, and 5’ and 3’ untranslated regions. The ES cells derived from 129 carrying targeting vector was electroporated into C57BL/6J blastocysts. The Chimeric mice were crossed with 129/Sv. The offspring were maintained in 129/Sv background.
3.  Mice homozygous for this allele are viable but infertile.
4.  Heterozygous and homozygous mice were observed growth retardation, disproportionate shortening of the limbs, round head, mid-face hypoplasia at birth, and kyphosis progression during development, the phenotype shown in achondroplasia (ACH) patients. Premature fusion of the cranial sutures and low bone density in newborn mice was also observed. The phenotype above mentioned is dosage-dependent.
5.  Soften the food and place on the cage floor is required during maintaining this strain.
6.   The suggested breeding strategies of this strain are (A) heterozygote * heterozygote and (B) heterozygote (male) * 129 (the background strain) (female).
     (1) The offspring from strategy (A) could be used for tests or experiments.
          The offspring from strategy (B) is to be breeders.
     (2) The phenotype will be less and less significant if the heterozygous breeders are                          from  the littermates or offspring of the strategy (A) mentioned above.
7.  Due to the teeth protruding of ACH mice, their teeth need regular trimming, it is better to put the soaked food at the bottom of the cage.
Depositor ：
Yi-Ching Lee / 李宜靜
Email ： yiching@gate.sinica.edu.tw
Allele Symbol ： Fgfr3tm1.1(FGFR3*)Ytc
Allele Name ： fibroblast growth factor receptor 3, target mutation 1.1, Yi-Ching Lee
Allele Description ：
Strain of Origin ： 129
Gene Symbol ： Fgfr3
Gene Name ： fibroblast growth factor receptor 3
Chromosome ： 5
Genotyping Method ： Download            
Genotyping NLAC ：            
Research Application ：
Cell and Developmental Biology Metabolism
Pharmacology and Toxicology Endocrine
Construction Technologies ：
Cre-lox system FLP-FRT system
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： hetero/hemi x wild type
References ： PubMed ID: 28230213
PubMed ID: 33784257
Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： Knock-in human FGFR3 achondroplasia mutation as a mouse model for human skeletal dysplasia. Sci Rep. 2017; 7: 43220
Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： Knock-in human FGFR3 achondroplasia mutation as a mouse model for human skeletal dysplasia. Sci Rep. 2017; 7: 43220
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由Dr. Yi-Ching Lee所提供
Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge Dr. Yi-Ching Lee
分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: yiching@gate.sinica.edu.tw
Construct Map ：
 
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