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RMRC13386
RMRC No.
RMRC13386
Order
Strain/Stock Designation
C57BL/6-Tg(Pax8-emGFP,-Tet-On 3G ; PTRE3G-cre)1Tmc/Narl
Status
EM
|
(Cryopreserved Embryos)
Availability
Level4
Pricing
(Embryos) Industry:11,105NTD Academia: 7,775NTD NSTC: 5,415NTD(Cryo Recovery) Industry:20,815NTD Academia: 13,880NTD NSTC: 13,880NTD
Last Update Date
2023/8/11
Analysis Data
Description
Genes &
alleles
Phenotype
Health &
husbandry
References
Specific Term
and Conditions
Construct map
Species
:
laboratory mouse
Strain Type
:
COI (coisogenic strain),MSR (mutant strain)
Mutant Type
:
TG (Transgenic)
Other Names
:
Pax8-cre GFP
Background Strain
:
C57BL/6
Strain Description
:
1.
The BAC-based transgene was designed with emGFP-IRES-Tet-on 3G (Doxycyline-responsive element Tet-on 3G) coding cassette in exon ENSMUSE00001317385 and cre controlled by PTRE3G (tetracycline response element promoter) downstream of ENSMUSE00000269274 of paired box 8 (
Pax8
). This transgene was microinjected into fertilized C57BL/6J pronuclei.
2.
The constitutive expression of emGFP and Tet-on 3G were under the control of
pax8
gene. The inducible promoter PTRE3G provides for very low basal expression of cre recombinase and high maximal expression after Doxycylineinduction.
Depositor
:
Chu Tang-Yuan
/
朱堂元
Email
:
Allele Symbol
:
Tg(Pax8-emGFP,-Tet-On 3G ; PTRE3G-cre)1Tmc
Allele Name
:
transgenic insertion 1, Chu Tang Yuan
Allele Description
:
Strain of Origin
:
C57BL/6
Gene Symbol
:
Pax8
Gene Name
:
paired box 8
Chromosome
:
UN
Genotyping Method
:
Download
Genotyping NLAC
:
Research Application
:
Cancer
Reproductive Biology
Construction Technologies
:
Tet Expression system
fluorescent proteins
Animal Health Reports
:
Colony Maintenance
Breeding & Husbandry
:
Colony Maintenance
Mating System
:
hetero/hemi x hetero/hemi
References
:
No
Specific Terms of Use
:
無特別規定
寄存者對於分讓申請人使用本材料所獲得之研究成果,不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE.
Construct Map
:
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