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RMRC No. RMRC13352 Order
Strain/Stock Designation C57BL/6-Septin12tm1(S196E)Kpln/Narl
Status
EM| (Cryopreserved Embryos)
Availability Level4
Pricing (Embryos) 　Industry：11,105NTD　Academia: 7,775NTD　NSTC: 5,415NTD
(Cryo Recovery) 　Industry：20,815NTD　Academia: 13,880NTD　NSTC: 13,880NTD
Last Update Date 2019/3/29
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： COI (coisogenic strain),MSR (mutant strain)
Mutant Type ： TM (Target Mutation)
Other Names ：
Background Strain ： C57BL/6
Strain Description ：
1.  The entire Septin12 genomic fragment in the bMQ251k06 BAC clone was obtained from the BACPAC Resource Center, and the fragment containing exon 2 to exon 10 was cloned into the pL253 targeting vector. A targeting vector with a serine-to-glutamate (S196E) substitution was introduced into exon 6 through site-directed mutagenesis, and loxP-neo-loxP cassette was inserted between exon 7 and exon 8 of Sept12. The target construct was electroporated into mouse embryonic stem cells to replace the wild-type allele ofSept12. Cell clones with the targeted allele were transfected with the cre-recombinase-expressing plasmid to remove the loxP-flanked neo cassette.
2. 
The homozygous knock-in mice displayed poor male fertility due to deformed sperm with defective motility and loss of annulus, a septin-based ring structure. It is suggested thatSept12 phosphorylation inhibits septin ring assembly during annulus biogenesis.
Depositor ：
Pao-Lin Kuo / 郭保麟
Email ： paolinkuo@gmail.com
Allele Symbol ： Septin12tm1(S196E)Kpln
Allele Name ： septin 12, target mutation 1, Pao-Lin Kuo
Allele Description ：
Strain of Origin ： C57BL/6
Gene Symbol ： Sept12
Gene Name ： septin 12
Chromosome ： 16
Genotyping Method ： Download            
Genotyping NLAC ：            
Research Application ：
Reproductive Biology
Construction Technologies ： No
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： hetero/hemi x hetero/hemi
References ： PubMed ID: 28346465

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility
Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility
分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: paolinkuo@gmail.com
Construct Map ：
 
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