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RMRC No. RMRC23007 Order
Strain/Stock Designation SD-Prkdcem1Narl/Narl
Status
EM| (Cryopreserved Embryos)
Availability Level4
Pricing
Last Update Date 2026/1/15
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory rat
Strain Type ：
Mutant Type ： TM (Target Mutation)
Other Names ： SD-Prkdcem1SCID/Narl
Background Strain ： SD
Strain Description ：
1.  A 4 bp substitution from “cggg” to “ttaa” in the first exon of Prkdc gene on rat chromosome 11q23 was introduced by CRISPR/Cas9 endonuclease originally in SHR embryos. This substitution generated a nonsense (taa) and theoretically null mutation at the 29th codon that resulted in the early termination in the translation process of the Prkdc protein, which is originally 4126 a.a. long.
2.  A heterozygous founder was selected for breeding with regular wild-type SD rats for multiple generations to dilute potential off-target effects and introduce genetic heterogeneity into this strain. Therefore, the foundation level ofanimals must be kept with such mating to select heterozygous carriers under the SD rat background.
3.  Homozygous rats are subsequently generated by sibling crossing of the heterozygous carriers, which appear physiologically normal; therefore, genotyping is required for every litter. While severe combined immunodeficiency (SCID) phenotype is found in homozygotes, fertility of homozygous rats has not been tested, but it can be expected as an option in subsequent colonies because wild-type or heterozygous littermates serve as excellent control cohorts matched for both age and sex to ensure stringent comparisons.
Depositor ：
National Center of Biomodels, National Institutes of Applied Research / 財團法人國家實驗研究院國家生物模式中心
Email ：
Allele Symbol ： Prkdcem1Narl
Allele Name ： protein kinase, DNA activated, catalytic polypeptide,endonuclease-mediated mutation 1, National Applied Research Laboratories
Allele Description ：
Strain of Origin ： SHR
Gene Symbol ：
Gene Name ：
Chromosome ：
Gene Name ： protein kinase, DNA activated, catalytic polypeptide
Chromosome ： 16
Genotyping Method ： Download            
Genotyping NLAC ：            
Research Application ：
Hematology Cancer
Immunology and Inflammation
Construction Technologies ： No
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： hetero/hemi x hetero/hemi
References ： No

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由財團法人國家實驗研究院國家生物模式中心所提供
Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge NCB, NIAR
分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email:
Construct Map ：
 
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