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RMRC No. RMRC13434 Order
Strain/Stock Designation C57BL/6-Tg(Mbl2,-cre, CAG-emGFP)11Narl/Narl
Status
SP| (Cryopreserved or freeze-dried sperm)
Availability Level4
Pricing Industry：23,680NTD　Academia: 23,680NTD　NSTC: 16,580NTD
Last Update Date 2026/2/2
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： COI (coisogenic strain),MSR (mutant strain)
Mutant Type ： TG (Transgenic)
Other Names ：
Background Strain ： C57BL/6
Strain Description ：
1.  A transgenic construct based on a bacterial artificial chromosome containing the full Mbl2 locus was engineered by inserting an IRES-Cre-polyA cassette into the last exon, along with a GFP reporter gene driven by a ubiquitous promoter (CAG) downstream of the 3’ end. Two transgenic mouse lines were generated via pronuclear microinjection and embryo transplantation, both of which expressed GFP.
2.  The Cre expression pattern was analyzed by breeding these mice with the Cre-reporter,C57BL/6-Tg(Actb-GFP,-FusionRed)10Narl/Narl, which expresses FusionRed after Cre-mediated excision of a floxed GFP cassette. As expected, red fluorescence derived from FusionRed expression was observed only in the liver of this Cre transgenic line #11. FusionRed-expressing cell types in the double transgenic livers were further examined by fluorescence microscopy, revealing the characteristic histological configuration of hepatocytes. To quantify the percentage of cells with an excised allele in various organs, we performed semi-quantitative PCR using two sets of primers that amplify the floxed and excised alleles, respectively, in a single reaction. The data showed that 12.47%, 54.18%, 68.02%, and 68.47% of liver cells in 1-, 14-, 28-, and 56-day-old mice, respectively, expressed Cre recombinase. The increasing Cre activity over time may reflect hepatocyte maturation, adding another layer of biological insight. The PCR product corresponding to the excised allele was not detected in any other organs examined, except for the testis. These results indicate that this Cretransgenic mice are useful tools for investigating gene function specifically in mature hepatocytes, and they may offer more accurate hepatocyte-specific gene control than traditional lines such as Albumin-Cre.
Depositor ：
National Center of Biomodels, National Institutes of Applied Research / 財團法人國家實驗研究院國家生物模式中心
Email ： bujiii@narlabs.org.tw
Allele Symbol ： Tg(Mbl2,-cre, CAG-emGFP)11Narl
Allele Name ： mannose-binding lectin (protein C) 2, Transgenic insertion 11, National Applied Research Laboratories
Allele Description ：
Strain of Origin ： C57BL/6
Gene Symbol ： Mbl2
Gene Name ： mannose-binding lectin (protein C) 2
Chromosome ： UN
Genotyping Method ： Download            
Genotyping NLAC ：            
Research Application ：
Internal/ Organ Cell and Developmental Biology
Construction Technologies ：
Cre-lox system fluorescent proteins
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： hetero/hemi x wild type
References ： No

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由NIAR- NCB所提供
Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge NIAR- NCB
Construct Map ：
 
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