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RMRC No. RMRC13410 Order
Strain/Stock Designation C57BL/6-Wdr19tm1Narl/Narl
Status
SP| (Cryopreserved or freeze-dried sperm)
Availability Level4
Pricing Industry：32,340NTD　Academia: 32,340NTD　NSTC: 22,640NTD
Last Update Date 2023/8/14
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： COI (coisogenic strain),MSR (mutant strain)
Mutant Type ： TM (Target Mutation)
Other Names ： Wdr19 floxed
Background Strain ： C57BL/6
Strain Description ：
1.  The cassette containingloxP-Wdr19 cDNA (E4-E36)-RFP-polyA tail-Frt-Neo-Frt-loxP replacedWdr19 regions from exon 4 to exon 37 in BAC (bacterial artificial chromosome) clone by homologous recombination. The linear modified BAC DNA was electroporated into C57BL/6JNarl ES cells (embryonic stem cells). The positive ES cells were injected into C57BL/6JNarl albino blastocysts which were transferred into pseudopregnant mice to generated chimeric mice.
2.  The Wdr19-KO mice (Wdr19f/- ; Cdh16-creTg/0) exhibited smaller bodies and larger kidneys than the WT (wild-type) mice at postnatal 21 days. The kidney weight-to-body ratio of Wdr19-KO mice was greater than that of their control littermates from postnatal 21 days to 28 days.
3.  The Wdr19-KO (knockout) mice exhibited very short or no primary cilia, while the WT and Pkd1-KO mice (Pkd1f/- ; Cdh16-creTg/0) exhibited intact primary cilia.
4.  The Wdr19 & Pkd1-KO mice (Wdr19f/- ; Pkd1f/- ; Cdh16-creTg/0) exhibited intermediated bodies and kidney sizes between the Wdr19-KO and Pkd1-KO mice at postnatal 14 days. Also, the renal cyst sizes of Wdr19 & Pkd1-KO mice were also between those of Wdr19-KO and Pkd1-KO mice.
Depositor ：
Si-Tse Jiang / 蔣思澈
Email ： stjiang@narlabs.org.tw
Allele Symbol ： Wdr19tm1Narl
Allele Name ： WD repeat domain 19,target mutation 1, National Applied Research Laboratories
Allele Description ：
Strain of Origin ： C57BL/6
Gene Symbol ： Wdr19
Gene Name ： WD repeat domain 19
Chromosome ： 5
Genotyping Method ： Download            
Genotyping NLAC ：            
Research Application ：
Internal/ Organ Cell and Developmental Biology
Construction Technologies ：
Cre-lox system
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： hetero/hemi x hetero/hemi
References ： PubMed ID: 35007346

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： J Pathol. 2022 257(1):5-16. Large deletion of Wdr19 in developing renal tubules disrupts primary ciliogenesis, leading to polycystic kidney disease in
Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： J Pathol. 2022 257(1):5-16. Large deletion of Wdr19 in developing renal tubules disrupts primary ciliogenesis, leading to polycystic kidney disease in mice
Construct Map ：
 
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